In vitro experiments

IN-HOUSE

Cellular health
  • Apoptosis/necrosis
  • DioC6 probe (flow cytometry)
  • 7AAD + annexin V (flow cytometry)
  • Mitochondrial activity
  • XTT conversion (spectrometry)
  • ATP measurement (luminometry)
  • Morphology
  • Microscopy
  • Proliferation
  • Cell count (image cytometry)
  • Cell cycle
  • G0/G1+S+G2/M sub-populations numbering (flow cytometry)
  • Oxidative stress
  • DAF and DCF family probes (global oxidative stress or specifically nitric oxide, fluorometry or flow cytometry)
  • Gene modulation (Q-PCR)
  • Cellular stress
  • Stress genes (or their end-products) modulation (Q-PCR, ELISA)
  • Membrane integrity
  • Trypan blue (microscopy)
  • LDH activity measurement (spectrometry)
  • 7AAD/IP (flow cytometry)
Genotoxicity
  • Ames test
  • Micronucleus assay
  • Repair/cell cycle genes modulation
  • Range of genes such as bax, p21, p53, mdm2, gadd45 (Q-PCR)
  • DNA adducts
  • P32 post-labelling
  • DNA Fragmentation
  • (TUNEL)
  • DNA denaturation
  • (chromomycin, aniline blue)
  • Aneuploidy analysis
  • (FISH)
  • Karyotype / chromosomal aberrations
  • number and structure anomaly
  • Molecular karyotyping
  • (genomic chip -low or high resolutions)
  • Mutation and fragment analysis
  • (PCR et sequence)
  • Expression profile analysis
  • (chip expression)
  • Tumerogenicity
  • (hetero- and orthotopic xenografts in immunosuppressed rats and mice)
Blood-Brain Barrier
  • Cellular health assessment
  • Permeability measurement
  • Kinetics of passage
  • Cerebral endothelium characterization
  • Paracellular diffusion
  • Transport proteins regulation
Angiogenesis
  • « Wound healing » on cellular monolayers
  • Invasion and/or migration through Boyden chambers
  • Boyden chambers
Inflammation
  • Transendothelial lymphocyte passage
  • Adhesion molecules expression
inhalation
  • In vitro experiments with direct exposition to the test atmosphere
  • In vivo biomonitoring on exposed populations (e.g.. : occupational health )

PARTNERSHIP

Transmembrane/organ passage
  • Skin
  • Intestine
  • Cornea
  • Mucosa
Endocrine disruptors
  • Steroïd disruptors
  • Interactions with enzyme receptors
  • Thyroïdian disruptors
  • Activity modulation of D2 and D3 enzymes
Metabolisation
  • Expression (inhibition/induction) of cytochromes
  • CYP
  • Metabolic stability
  • Characterisation of metabolites
Reprotoxicity
  • Follicle biossay
  • In vitro maturation assay
  • in vitro fertilization assay
  • mouse embryo assay
  • embryonic stem cell test
  • uterine contractivity
  • sperm analysis